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KMID : 0545119960060010019
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Journal of Microbiology and Biotechnology
1996 Volume.6 No. 1 p.19 ~ p.25
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Purification and Characteristics of Chitosanase from Bacillus sp. HW-002
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LEE, HYEAN-WOO
CHOI, JONG-WHAN/HAN, DONG-PYOU/PARK, MYOUNG-JIN/LEE, NO-WOON/YI, DONG-HEUI
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Abstract
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Chitosanase from Bacillus sp. HW-002 was purified with CM-cellulose column chromatography, and HPLC with DEAE-TSK gel and YMC-park Diol 120. The purified enzyme appeared as a single band on SDS-polyacrylamide gel. The molecular weight of the enzyme was estimated to be about 46 kDa on SDS- polyacrylamide gel, and was estimated to be about 23 kDa by GFC. The optimal pH of chitosanolytic activity was about pH 5.5-6.0, and the purified enzyme was most stable at pH 5.0. The optimal temperature of chitosanolytic activity was 65℃ and the enzyme was stable at 45℃ for 1 h. Chitosan was the most favorable substrate among various β-glucan. UVmax of the purified enzyme was 195 nm and was not noted around 280 nm. The main product of enzyme reaction with chitosan was chitobiose.
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